| Saturday 2nd February 1889 | |||
| First Round | |||
| Accrington | 1-1 | Blackburn Rovers | |
| Aston Villa | 3-2 | Witton | |
| Birmingham City | 2-3 | West Bromwich Albion | |
| Birmingham St George | 3-2 | Long Eaton Rangers | |
| Bootle (old) | 0-3 | Preston North End | |
| Burnley | 4-3 | Old Westminsters | |
| Chatham | 2-1 | South Shore | aet |
| Derby County | 1-0 | Derby Junction | |
| Grimsby Town | 3-1 | Sunderland Albion | |
| Halliwell | 2-2 | Crewe Alexandra | aet |
| Nottingham Forest | 2-2 | Linfield Athletic | aet |
| Notts County | 2-0 | Old Brightonians | |
| Notts Rangers | 1-1 | Sheffield Wednesday | |
| Swifts | 3-1 | Wrexham | |
| Walsall | 5-1 | Sheffield Heeley | |
| Wolverhampton Wndrs | 4-3 | Old Carthusians | |
» English FA Cup 1888-1889 : Results
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