| Saturday 23rd January 2010 | |||
| Accrington Stanley | 1-3 | Fulham | |
| Aston Villa | 3-2 | Brighton and Hove A | |
| Bolton Wanderers | 2-0 | Sheffield United | |
| Cardiff City | 4-2 | Leicester City | |
| Derby County | 1-0 | Doncaster Rovers | |
| Everton | 1-2 | Birmingham City | |
| Notts County | 2-2 | Wigan Athletic | |
| Portsmouth | 2-1 | Sunderland | |
| Preston North End | 0-2 | Chelsea | |
| Reading | 1-0 | Burnley | |
| Southampton | 2-1 | Ipswich Town | |
| Tottenham Hotspur | 2-2 | Leeds United | |
| West Bromwich Albion | 4-2 | Newcastle United | |
| Wolverhampton Wndrs | 2-2 | Crystal Palace | |
| Sunday 24th January 2010 | |||
| Scunthorpe United | 2-4 | Manchester City | |
| Stoke City | 3-1 | Arsenal | |
| Tuesday 2nd February 2010 | |||
| Replay | |||
| Crystal Palace | 3-1 | Wolverhampton Wndrs | |
| Wigan Athletic | 0-2 | Notts County | |
| Wednesday 3rd February 2010 | |||
| Replay | |||
| Leeds United | 1-3 | Tottenham Hotspur | |
