| Friday 30th November 2012 | |||
| Bradford City | 1-1 | Brentford | |
| Saturday 1st December 2012 | |||
| Accrington Stanley | 3-3 | Oxford United | |
| Bury | 1-1 | Southend United | |
| Carlisle United | 1-3 | Bournemouth | |
| Coventry City | 2-1 | Morecambe | |
| Crawley Town | 3-0 | Chelmsford City | |
| Crewe Alexandra | 0-1 | Burton Albion | |
| Fleetwood Town | 2-3 | Aldershot Town | |
| Harrogate Town | 1-1 | Hastings United | |
| Lincoln City | 3-3 | Mansfield Town | |
| Luton Town | 2-1 | Dorchester Town | |
| Oldham Athletic | 3-1 | Doncaster Rovers | |
| Preston North End | 2-0 | Gillingham | |
| Rotherham United | 1-1 | Notts County | |
| Sheffield United | 2-1 | Port Vale | |
| Tranmere Rovers | 2-1 | Chesterfield | |
| Sunday 2nd December 2012 | |||
| Alfreton Town | 2-4 | Leyton Orient | |
| Milton Keynes Dons | 2-1 | AFC Wimbledon | |
| Monday 3rd December 2012 | |||
| Cheltenham Town | 1-1 | Hereford United | |
| Tuesday 11th December 2012 | |||
| Replay | |||
| Hereford United | 1-2 | Cheltenham Town | aet |
| Southend United | 1-1 | Bury | aet |
| (Southend United won on penalties) | |||
| Wednesday 12th December 2012 | |||
| Replay | |||
| Mansfield Town | 2-1 | Lincoln City | |
| Thursday 13th December 2012 | |||
| Replay | |||
| Hastings United | 1-1 | Harrogate Town | aet |
| (Hastings United won on penalties) | |||
| Tuesday 18th December 2012 | |||
| Barrow | 1-1 | Macclesfield Town | |
| Replay | |||
| Brentford | 4-2 | Bradford City | aet |
| Notts County | 0-3 | Rotherham United | |
| Oxford United | 2-0 | Accrington Stanley | |
| Saturday 29th December 2012 | |||
| Replay | |||
| Macclesfield Town | 4-1 | Barrow | |
