| Tuesday 28th September 2004 | |||
| Aldershot Town | 0-1 | Wycombe Wanderers | |
| Barnet | 3-1 | Stevenage | |
| Brentford | 0-3 | Milton Keynes Dons | |
| Bristol Rovers | 1-0 | Kidderminster Harr | |
| Cheltenham Town | 5-1 | Dagenham and Redb'ge | |
| Shrewsbury Town | 3-2 | Bournemouth | |
| Swansea City | 2-0 | Luton Town | |
| Torquay United | 4-3 | Yeovil Town | aet |
| Walsall | 1-0 | Rushden and Diamonds | |
| Woking | 0-3 | Leyton Orient | |
| Wednesday 29th September 2004 | |||
| Boston United | 0-1 | Cambridge United | |
| Bristol City | 1-0 | Peterborough United | |
| Colchester United | 1-1 | Southend United | aet |
| (Southend United won on penalties) | |||
| Oxford United | 2-2 | Exeter City | aet |
| (Exeter City won on penalties) | |||









