| Tuesday 24th August 2010 | |||
| Blackburn Rovers | 3-1 | Norwich City | |
| Bradford City | 1-2 | Preston North End | aet |
| Brentford | 2-1 | Hull City | |
| Crewe Alexandra | 0-1 | Ipswich Town | aet |
| Hartlepool United | 0-3 | Wigan Athletic | |
| Leeds United | 1-2 | Leicester City | |
| Leyton Orient | 0-2 | West Bromwich Albion | |
| Millwall | 2-1 | Middlesbrough | |
| Milton Keynes Dons | 4-3 | Blackpool | aet |
| Morecambe | 1-3 | Burnley | |
| Peterborough United | 2-1 | Cardiff City | |
| Portsmouth | 1-1 | Crystal Palace | aet |
| (Portsmouth won on penalties) | |||
| Reading | 3-3 | Northampton Town | aet |
| (Northampton Town won on penalties) | |||
| Scunthorpe United | 4-2 | Sheffield Wednesday | |
| Southampton | 0-1 | Bolton Wanderers | |
| Stoke City | 2-1 | Shrewsbury Town | |
| Sunderland | 2-0 | Colchester United | |
| Tranmere Rovers | 1-3 | Swansea City | |
| Watford | 1-2 | Notts County | |
| West Ham United | 1-0 | Oxford United | |
| Wolverhampton Wndrs | 2-1 | Southend United | aet |
| Wednesday 25th August 2010 | |||
| Accrington Stanley | 2-3 | Newcastle United | |
| Everton | 5-1 | Huddersfield Town | |
| Fulham | 6-0 | Port Vale | |
| Thursday 26th August 2010 | |||
| Birmingham City | 3-2 | Rochdale | |
