| Saturday 11th August 2012 | |||
| Bury | 1-2 | Middlesbrough | |
| Carlisle United | 1-0 | Accrington Stanley | |
| Cheltenham Town | 1-1 | Milton Keynes Dons | aet |
| (Milton Keynes Dons won on penalties) | |||
| Crewe Alexandra | 5-0 | Hartlepool United | |
| Doncaster Rovers | 1-1 | York City | aet |
| (Doncaster Rovers won on penalties) | |||
| Hull City | 1-1 | Rotherham United | aet |
| (Hull City won on penalties) | |||
| Leeds United | 4-0 | Shrewsbury Town | |
| Notts County | 0-1 | Bradford City | aet |
| Rochdale | 3-4 | Barnsley | aet |
| Sheffield United | 2-2 | Burton Albion | aet |
| (Burton Albion won on penalties) | |||
| Walsall | 1-0 | Brentford | |
| Watford | 1-0 | Wycombe Wanderers | aet |
| Wolverhampton Wndrs | 1-1 | Aldershot Town | aet |
| (Wolverhampton Wndrs won on penalties) | |||
| Sunday 12th August 2012 | |||
| Blackpool | 1-2 | Morecambe | |
| Monday 13th August 2012 | |||
| Fleetwood Town | 0-1 | Nottingham Forest | |
| Oldham Athletic | 2-4 | Sheffield Wednesday | |
| Preston North End | 2-0 | Huddersfield Town | |
| Tuesday 14th August 2012 | |||
| Birmingham City | 5-1 | Barnet | |
| Bristol City | 1-2 | Gillingham | |
| Charlton Athletic | 1-1 | Leyton Orient | aet |
| (Leyton Orient won on penalties) | |||
| Chesterfield | 1-2 | Tranmere Rovers | aet |
| Dagenham and Redb'ge | 0-1 | Coventry City | |
| Derby County | 5-5 | Scunthorpe United | aet |
| (Scunthorpe United won on penalties) | |||
| Exeter City | 1-2 | Crystal Palace | |
| Ipswich Town | 3-1 | Bristol Rovers | |
| Millwall | 2-2 | Crawley Town | aet |
| (Crawley Town won on penalties) | |||
| Northampton Town | 2-1 | Cardiff City | |
| Oxford United | 0-0 | Bournemouth | aet |
| (Oxford United won on penalties) | |||
| Peterborough United | 4-0 | Southend United | |
| Plymouth Argyle | 3-0 | Portsmouth | |
| Port Vale | 1-3 | Burnley | |
| Stevenage | 3-1 | AFC Wimbledon | |
| Swindon Town | 3-0 | Brighton and Hove A | |
| Torquay United | 0-4 | Leicester City | |
| Yeovil Town | 3-0 | Colchester United | |
