| Tuesday 13th July 2010 | |||
| 1st Leg | |||
| AIK Stockholm | 1-0 | Jeunesse Esch | |
| Birkirkara | 1-0 | MSK Zilina | |
| Bohemians | 1-0 | The New Saints | |
| Dinamo Zagreb | 5-1 | FC Koper | |
| Hapoel Tel-Aviv | 5-0 | Zeljeznicar Sarajevo | |
| Inter Baku | 0-1 | Lech Poznan | |
| Levadia Tallinn | 1-1 | Debreceni VSC | |
| Liteks Lovech | 1-0 | FK Rudar Pljevlja | |
| Metalurgs Liepajas | 0-3 | Sparta Prague | |
| Omonia Nicosia | 3-0 | Renova | |
| Salzburg | 5-0 | HB Torshavn | |
| Wednesday 14th July 2010 | |||
| 1st Leg | |||
| BATE Borisov | 5-1 | FH Hafnarfjordur | |
| FK Aktobe Lento | 2-0 | Metalurgi Rustavi | |
| FK Ekranas Panevezys | 1-0 | HJK Helsinki | |
| Linfield | 0-0 | Rosenborg | |
| Partizan Belgrade | 3-1 | Pyunik Yerevan | |
| Sheriff Tiraspol | 3-1 | Dinamo Tirana | |
| Tuesday 20th July 2010 | |||
| 2nd Leg | |||
| Dinamo Tirana | 1-0 | Sheriff Tiraspol | (2-3) |
| FC Koper | 3-0 | Dinamo Zagreb | (4-5) |
| FK Rudar Pljevlja | 0-4 | Liteks Lovech | (0-5) |
| HB Torshavn | 1-0 | Salzburg | (1-5) |
| Renova | 0-2 | Omonia Nicosia | (0-5) |
| The New Saints | 4-0 | Bohemians | (4-1) |
| Wednesday 21st July 2010 | |||
| 2nd Leg | |||
| Debreceni VSC | 3-2 | Levadia Tallinn | (4-3) |
| FH Hafnarfjordur | 0-1 | BATE Borisov | (1-6) |
| HJK Helsinki | 2-0 | FK Ekranas Panevezys | aet (2-1) |
| Jeunesse Esch | 0-0 | AIK Stockholm | (0-1) |
| Lech Poznan | 0-1 | Inter Baku | aet (1-1) |
| (Lech Poznan won on penalties) | |||
| Metalurgi Rustavi | 1-1 | FK Aktobe Lento | (1-3) |
| MSK Zilina | 3-0 | Birkirkara | (3-1) |
| Pyunik Yerevan | 0-1 | Partizan Belgrade | (1-4) |
| Rosenborg | 2-0 | Linfield | (2-0) |
| Sparta Prague | 2-0 | Metalurgs Liepajas | (5-0) |
| Zeljeznicar Sarajevo | 0-1 | Hapoel Tel-Aviv | (0-6) |
