| Tuesday 27th July 2010 | |||
| 1st Leg | |||
| Dinamo Kiev | 3-0 | KAA Gent | |
| Liteks Lovech | 1-1 | MSK Zilina | |
| Omonia Nicosia | 1-1 | Salzburg | |
| Sparta Prague | 1-0 | Lech Poznan | |
| The New Saints | 1-3 | Anderlecht | |
| Unirea Urziceni | 0-0 | Zenit St Petersburg | |
| Wednesday 28th July 2010 | |||
| 1st Leg | |||
| AIK Stockholm | 0-1 | Rosenborg | |
| Ajax | 1-1 | PAOK Salonika | |
| BATE Borisov | 0-0 | FC Copenhagen | |
| Debreceni VSC | 0-2 | Basle | |
| FK Aktobe Lento | 1-0 | Hapoel Tel-Aviv | |
| Partizan Belgrade | 3-0 | HJK Helsinki | |
| Sheriff Tiraspol | 1-1 | Dinamo Zagreb | |
| Sporting Braga | 3-0 | Celtic | |
| Young Boys | 2-2 | Fenerbahce | |
| Tuesday 3rd August 2010 | |||
| 2nd Leg | |||
| Anderlecht | 3-0 | The New Saints | (6-1) |
| Hapoel Tel-Aviv | 3-1 | FK Aktobe Lento | (3-2) |
| Wednesday 4th August 2010 | |||
| 2nd Leg | |||
| Basle | 3-1 | Debreceni VSC | (5-1) |
| Celtic | 2-1 | Sporting Braga | (2-4) |
| Dinamo Zagreb | 1-1 | Sheriff Tiraspol | aet (2-2) |
| (Sheriff Tiraspol won on penalties) | |||
| FC Copenhagen | 3-2 | BATE Borisov | (3-2) |
| Fenerbahce | 0-1 | Young Boys | (2-3) |
| HJK Helsinki | 1-2 | Partizan Belgrade | (1-5) |
| KAA Gent | 1-3 | Dinamo Kiev | (1-6) |
| Lech Poznan | 0-1 | Sparta Prague | (0-2) |
| MSK Zilina | 3-1 | Liteks Lovech | (4-2) |
| PAOK Salonika | 3-3 | Ajax | (4-4) |
| (Ajax won on away goals) | |||
| Rosenborg | 3-0 | AIK Stockholm | (4-0) |
| Salzburg | 4-1 | Omonia Nicosia | (5-2) |
| Zenit St Petersburg | 1-0 | Unirea Urziceni | (1-0) |
