| Tuesday 17th August 2010 | |||
| 1st Leg | |||
| Dinamo Kiev | 1-1 | Ajax | |
| Rosenborg | 2-1 | FC Copenhagen | |
| Sparta Prague | 0-2 | MSK Zilina | |
| Young Boys | 3-2 | Tottenham Hotspur | |
| Zenit St Petersburg | 1-0 | Auxerre | |
| Wednesday 18th August 2010 | |||
| 1st Leg | |||
| Basle | 1-0 | Sheriff Tiraspol | |
| Partizan Belgrade | 2-2 | Anderlecht | |
| Salzburg | 2-3 | Hapoel Tel-Aviv | |
| Sporting Braga | 1-0 | Seville | |
| Werder Bremen | 3-1 | Sampdoria | |
| Tuesday 24th August 2010 | |||
| 2nd Leg | |||
| Anderlecht | 2-2 | Partizan Belgrade | aet (4-4) |
| (Partizan Belgrade won on penalties) | |||
| Hapoel Tel-Aviv | 1-1 | Salzburg | (4-3) |
| Sampdoria | 3-2 | Werder Bremen | aet (4-5) |
| Seville | 3-4 | Sporting Braga | (3-5) |
| Sheriff Tiraspol | 0-3 | Basle | (0-4) |
| Wednesday 25th August 2010 | |||
| 2nd Leg | |||
| Ajax | 2-1 | Dinamo Kiev | (3-2) |
| Auxerre | 2-0 | Zenit St Petersburg | (2-1) |
| FC Copenhagen | 1-0 | Rosenborg | (2-2) |
| (FC Copenhagen won on away goals) | |||
| MSK Zilina | 1-0 | Sparta Prague | (3-0) |
| Tottenham Hotspur | 4-0 | Young Boys | (6-3) |
