| Thursday 25th February 2010 | |||
| Second Round 2nd Leg | |||
| Anderlecht | 4-0 | Athletic Bilbao | (5-1) |
| Fenerbahce | 1-1 | Lille | (2-3) |
| Galatasaray | 1-2 | Atletico Madrid | (2-3) |
| Hapoel Tel-Aviv | 0-0 | Rubin Kazan | (0-3) |
| Juventus | 0-0 | Ajax | (2-1) |
| Marseille | 3-1 | FC Copenhagen | (6-2) |
| PSV Eindhoven | 3-2 | SV Hamburg | (3-3) |
| Roma | 2-3 | Panathinaikos | (4-6) |
| Salzburg | 0-0 | Standard Liege | (2-3) |
| Shakhtar Donetsk | 1-1 | Fulham | (2-3) |
| Sporting Lisbon | 3-0 | Everton | (4-2) |
| Unirea Urziceni | 1-3 | Liverpool | (1-4) |
| Valencia | 3-0 | Club Brugge | aet (3-1) |
| VfL Wolfsburg | 4-1 | Villarreal | (6-3) |
| Werder Bremen | 4-1 | FC Twente Enschede | (4-2) |
| Thursday 11th March 2010 | |||
| Third Round 1st Leg | |||
| Atletico Madrid | v | Sporting Lisbon | 18:00 |
| Benfica | v | Marseille | 20:05 |
| Juventus | v | Fulham | 20:05 |
| Lille | v | Liverpool | 18:00 |
| Panathinaikos | v | Standard Liege | 20:05 |
| Rubin Kazan | v | VfL Wolfsburg | 18:00 |
| SV Hamburg | v | Anderlecht | 18:00 |
| Valencia | v | Werder Bremen | 20:05 |
