| Tuesday 16th February 2010 | |||
| 1st Leg | |||
| Everton | 2-1 | Sporting Lisbon | |
| Thursday 18th February 2010 | |||
| 1st Leg | |||
| Ajax | 1-2 | Juventus | |
| Athletic Bilbao | 1-1 | Anderlecht | |
| Atletico Madrid | 1-1 | Galatasaray | |
| Club Brugge | 1-0 | Valencia | |
| FC Copenhagen | 1-3 | Marseille | |
| FC Twente Enschede | 1-0 | Werder Bremen | |
| Fulham | 2-1 | Shakhtar Donetsk | |
| Hertha BSC Berlin | 1-1 | Benfica | |
| Lille | 2-1 | Fenerbahce | |
| Liverpool | 1-0 | Unirea Urziceni | |
| Panathinaikos | 3-2 | Roma | |
| Rubin Kazan | 3-0 | Hapoel Tel-Aviv | |
| Standard Liege | 3-2 | Salzburg | |
| SV Hamburg | 1-0 | PSV Eindhoven | |
| Villarreal | 2-2 | VfL Wolfsburg | |
| Tuesday 23rd February 2010 | |||
| 2nd Leg | |||
| Benfica | 4-0 | Hertha BSC Berlin | (5-1) |
| Thursday 25th February 2010 | |||
| 2nd Leg | |||
| Anderlecht | 4-0 | Athletic Bilbao | (5-1) |
| Fenerbahce | 1-1 | Lille | (2-3) |
| Galatasaray | 1-2 | Atletico Madrid | (2-3) |
| Hapoel Tel-Aviv | 0-0 | Rubin Kazan | (0-3) |
| Juventus | 0-0 | Ajax | (2-1) |
| Marseille | 3-1 | FC Copenhagen | (6-2) |
| PSV Eindhoven | 3-2 | SV Hamburg | (3-3) |
| Roma | 2-3 | Panathinaikos | (4-6) |
| Salzburg | 0-0 | Standard Liege | (2-3) |
| Shakhtar Donetsk | 1-1 | Fulham | (2-3) |
| Sporting Lisbon | 3-0 | Everton | (4-2) |
| Unirea Urziceni | 1-3 | Liverpool | (1-4) |
| Valencia | 3-0 | Club Brugge | aet (3-1) |
| VfL Wolfsburg | 4-1 | Villarreal | (6-3) |
| Werder Bremen | 4-1 | FC Twente Enschede | (4-2) |
February 2010
| Jul | Aug | Sep | Oct | Nov | Dec |
| Jan | Feb | Mar | Apr | May | Jun |
| Mo | Tu | We | Th | Fr | Sa | Su |
| 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| 15 | 16 | 17 | 18 | 19 | 20 | 21 |
| 22 | 23 | 24 | 25 | 26 | 27 | 28 |
- Final
- Semi-Final
- Quarter-Final
- Third Round
- Second Round
- First Round Group A
- First Round Group B
- First Round Group C
- First Round Group D
- First Round Group E
- First Round Group F
- First Round Group G
- First Round Group H
- First Round Group I
- First Round Group J
- First Round Group K
- First Round Group L
- Qualifying Play-Off
- Third Qualifying Round
- Second Qualifying Round
- First Qualifying Round
