| Saturday 25th September 2010 | |||
| Beith | 2-0 | Linlithgow Rose | |
| Civil Service Str | 1-2 | Wigtown and Bladnoch | |
| Coldstream | 1-3 | Forres Mechanics | |
| Deveronvale | 0-0 | Inverurie Locos | |
| Edinburgh City | 1-0 | Clachnacuddin | |
| Edinburgh University | 2-2 | Brora Rangers | |
| Fraserburgh | 3-3 | St Cuthberts Wndrs | |
| Gala Fairydean | 1-6 | Sunnybank | |
| Glasgow University | 1-0 | Burntisland Shipyard | |
| Golspie Sutherland | 2-2 | Fort William | |
| Hawick Royal Albert | 0-3 | Dalbeattie Star | |
| Huntly | 2-2 | Girvan | |
| Lossiemouth | 0-2 | Whitehill Welfare | |
| Newton Stewart Ath | 1-1 | Preston Athletic | |
| Rothes | 2-2 | Nairn County | |
| Selkirk | 1-6 | Bo'ness United | |
| Vale of Leithen | 1-3 | Keith | |
| Saturday 2nd October 2010 | |||
| Replay | |||
| Brora Rangers | 2-1 | Edinburgh University | |
| Fort William | 2-3 | Golspie Sutherland | |
| Girvan | 2-1 | Huntly | |
| Inverurie Locos | 0-5 | Deveronvale | |
| Nairn County | 4-1 | Rothes | |
| Preston Athletic | 3-0 | Newton Stewart Ath | |
| St Cuthberts Wndrs | 3-1 | Fraserburgh | |
