| Saturday 25th August 2012 | |||
| Bonnyrigg Rose | 2-1 | Girvan | |
| Buckie Thistle | 0-0 | Rothes | |
| Civil Service Str | 4-0 | Newton Stewart Ath | |
| Clachnacuddin | 2-1 | Lossiemouth | |
| Edinburgh University | 1-2 | St Cuthberts Wndrs | |
| Formartine United | 3-2 | Brora Rangers | |
| Fraserburgh | 4-0 | Coldstream | |
| Glasgow University | 0-2 | Selkirk | |
| Hawick Royal Albert | 2-4 | Golspie Sutherland | |
| Hermes | 1-4 | Deveronvale | |
| Huntly | 2-2 | Wigtown and Bladnoch | |
| Irvine Meadow | 4-0 | Gala Fairydean | |
| Preston Athletic | 0-2 | Nairn County | |
| Shotts Bon Accord | 1-1 | Edinburgh City | |
| Spartans | 0-2 | Wick Academy | |
| Threave Rovers | 0-1 | Vale of Leithen | |
| Turriff United | 6-1 | Burntisland Shipyard | |
| Whitehill Welfare | 2-4 | Inverurie Locos | |
| Saturday 1st September 2012 | |||
| Replay | |||
| Edinburgh City | 4-1 | Shotts Bon Accord | |
| Rothes | 0-4 | Buckie Thistle | |
| Wigtown and Bladnoch | 0-2 | Huntly | |
