| Friday 8th August 1980 | |||
| 1st Leg | |||
| Lincoln City | 5-0 | Hull City | |
| Saturday 9th August 1980 | |||
| 1st Leg | |||
| Aldershot | 2-0 | Wimbledon | |
| Blackburn Rovers | 0-0 | Huddersfield Town | |
| Bournemouth | 1-1 | Swindon Town | |
| Brentford | 3-1 | Charlton Athletic | |
| Bury | 2-2 | Halifax Town | |
| Carlisle United | 2-0 | Rochdale | |
| Chester City | 1-1 | Stockport County | |
| Chesterfield | 1-0 | Darlington | |
| Colchester United | 0-2 | Gillingham | |
| Doncaster Rovers | 1-1 | Mansfield Town | |
| Exeter City | 1-1 | Bristol Rovers | |
| Grimsby Town | 1-0 | Notts County | |
| Hereford United | 1-0 | Newport County | |
| Northampton Town | 0-2 | Reading | |
| Peterborough United | 3-2 | Fulham | |
| Plymouth Argyle | 0-1 | Portsmouth | |
| Port Vale | 2-3 | Tranmere Rovers | |
| Rotherham United | 1-3 | Bradford City | |
| Scunthorpe United | 0-1 | Barnsley | |
| Sheffield Wednesday | 2-0 | Sheffield United | |
| Southend United | 1-0 | Oxford United | |
| Torquay United | 0-0 | Cardiff City | |
| Walsall | 2-3 | Blackpool | |
| Watford | 2-1 | Millwall | |
| Wigan Athletic | 2-1 | Crewe Alexandra | |
| Wrexham | 1-3 | Burnley | |
| York City | 2-1 | Hartlepool United | |
| Monday 11th August 1980 | |||
| 2nd Leg | |||
| Stockport County | 1-0 | Chester City | aet (2-1) |
| Tuesday 12th August 1980 | |||
| 2nd Leg | |||
| Barnsley | 2-1 | Scunthorpe United | (3-1) |
| Bristol Rovers | 1-1 | Exeter City | aet (2-2) |
| (Bristol Rovers won on penalties) | |||
| Burnley | 2-1 | Wrexham | (5-2) |
| Charlton Athletic | 5-0 | Brentford | (6-3) |
| Darlington | 1-2 | Chesterfield | (1-3) |
| Fulham | 1-1 | Peterborough United | aet (3-4) |
| Gillingham | 2-1 | Colchester United | (4-1) |
| Halifax Town | 0-1 | Bury | (2-3) |
| Hartlepool United | 0-0 | York City | (1-2) |
| Huddersfield Town | 1-1 | Blackburn Rovers | aet (1-1) |
| (Blackburn Rovers won on away goals) | |||
| Hull City | 0-2 | Lincoln City | (0-7) |
| Mansfield Town | 2-1 | Doncaster Rovers | aet (3-2) |
| Millwall | 0-2 | Watford | (1-4) |
| Newport County | 5-0 | Hereford United | (5-1) |
| Notts County | 3-0 | Grimsby Town | (3-1) |
| Portsmouth | 2-1 | Plymouth Argyle | (3-1) |
| Rochdale | 1-1 | Carlisle United | (1-3) |
| Sheffield United | 1-1 | Sheffield Wednesday | (1-3) |
| Swindon Town | 2-0 | Bournemouth | aet (3-1) |
| Tranmere Rovers | 0-1 | Port Vale | aet (3-3) |
| (Tranmere Rovers won on away goals) | |||
| Wimbledon | 4-1 | Aldershot | (4-3) |
| Wednesday 13th August 1980 | |||
| 2nd Leg | |||
| Blackpool | 3-1 | Walsall | (6-3) |
| Bradford City | 0-0 | Rotherham United | (3-1) |
| Cardiff City | 2-1 | Torquay United | (2-1) |
| Crewe Alexandra | 2-2 | Wigan Athletic | aet (3-4) |
| Oxford United | 2-0 | Southend United | (2-1) |
| Reading | 2-3 | Northampton Town | (4-3) |
