| Tuesday 9th August 2005 | |||
| 1st Leg | |||
| Anorthosis Famagusta | 1-2 | Rangers | |
| Everton | 1-2 | Villarreal | |
| Manchester United | 3-0 | Debreceni VSC | |
| Real Betis | 1-0 | Monaco | |
| Valerenga | 1-0 | Club Brugge | |
| Wisla Krakow | 3-1 | Panathinaikos | |
| Wednesday 10th August 2005 | |||
| 1st Leg | |||
| Anderlecht | 2-1 | Slavia Prague | |
| Artmedia Petrzalka | 0-0 | Partizan Belgrade | |
| Basle | 2-1 | Werder Bremen | |
| Brondby | 2-2 | Ajax | |
| CSKA Sofia | 1-3 | Liverpool | |
| Malmo | 0-1 | FC Thun | |
| Rapid Vienna | 1-1 | Lokomotiv Moscow | |
| Shakhtar Donetsk | 0-2 | Inter Milan | |
| Sporting Lisbon | 0-1 | Udinese | |
| Steaua Bucharest | 1-1 | Rosenborg | |
| Tuesday 23rd August 2005 | |||
| 2nd Leg | |||
| FC Thun | 3-0 | Malmo | (4-0) |
| Liverpool | 0-1 | CSKA Sofia | (3-2) |
| Lokomotiv Moscow | 0-1 | Rapid Vienna | (1-2) |
| Monaco | 2-2 | Real Betis | (2-3) |
| Panathinaikos | 4-1 | Wisla Krakow | aet (5-4) |
| Partizan Belgrade | 0-0 | Artmedia Petrzalka | aet (0-0) |
| (Artmedia Petrzalka won on penalties) | |||
| Rosenborg | 3-2 | Steaua Bucharest | (4-3) |
| Udinese | 3-2 | Sporting Lisbon | (4-2) |
| Wednesday 24th August 2005 | |||
| 2nd Leg | |||
| Ajax | 3-1 | Brondby | (5-3) |
| Club Brugge | 1-0 | Valerenga | aet (1-1) |
| (Club Brugge won on penalties) | |||
| Debreceni VSC | 0-3 | Manchester United | (0-6) |
| Inter Milan | 1-1 | Shakhtar Donetsk | (3-1) |
| Rangers | 2-0 | Anorthosis Famagusta | (4-1) |
| Slavia Prague | 0-2 | Anderlecht | (1-4) |
| Villarreal | 2-1 | Everton | (4-2) |
| Werder Bremen | 3-0 | Basle | (4-2) |
