| Tuesday 27th August 2013 | |||
| Barnsley | 1-5 | Southampton | |
| Bristol City | 2-1 | Crystal Palace | |
| Burnley | 2-0 | Preston North End | |
| Burton Albion | 2-2 | Fulham | aet |
| (Fulham won on penalties) | |||
| Carlisle United | 2-5 | Leicester City | |
| Derby County | 5-0 | Brentford | |
| Doncaster Rovers | 1-3 | Leeds United | |
| Huddersfield Town | 3-2 | Charlton Athletic | |
| Leyton Orient | 0-1 | Hull City | aet |
| Liverpool | 4-2 | Notts County | aet |
| Norwich City | 6-3 | Bury | |
| Peterborough United | 6-0 | Reading | |
| Queens Park Rangers | 0-2 | Swindon Town | |
| Sunderland | 4-2 | Milton Keynes Dons | |
| Tranmere Rovers | 1-1 | Bolton Wanderers | aet |
| (Tranmere Rovers won on penalties) | |||
| West Bromwich Albion | 3-0 | Newport County AFC | |
| West Ham United | 2-1 | Cheltenham Town | |
| Yeovil Town | 3-3 | Birmingham City | aet |
| (Birmingham City won on penalties) | |||
| Wednesday 28th August 2013 | |||
| Accrington Stanley | 0-2 | Cardiff City | |
| Aston Villa | 3-0 | Rotherham United | |
| Everton | 2-1 | Stevenage | aet |
| Morecambe | 0-2 | Newcastle United | |
| Nottingham Forest | 2-1 | Millwall | aet |
| Stoke City | 3-1 | Walsall | |
| Watford | 2-0 | Bournemouth | |
